Reagents for Real-time PCR

with TaqMan Probe & Intercalating Dye

Research Use Only. Not for Use in Diagnostic Procedures.

AddProbe qPCR Master (2x conc.) with UDG

for TaqMan Probe

For real-time PCR with TaqMan Probe

UDG (Uracil-DNA Glycosylase) for prevention of carryover contamination from amplification products is included in      AddProbe qPCR Master.

All components for real-time PCR is included – hot-start Taq DNA Polymerase, UDG, reaction buffer, dNTP mixture      and PCR enhancer.

Applications: Real-time PCR, Quantitative real-time PCR, Infectious Disease detection

qPCR B2M data.png

Test of Limit of Detection (LOD) between “AddBioMeditek” and “Competitive A qPCR Product” with 10-fold serial dilutions of human genomic DNA (10, 1, 0.1 and 0.01ng/rxn).

Target gene: human B2M                     

Square Line: AddBioMeditek, Linear Line: Competitive A

qPCR GAPDH data.png

Test of Limit of Detection (LOD) between “AddBioMeditek” and “Competitive A qPCR Product” with 10-fold serial dilutions of human genomic DNA (10, 1, 0.1 and 0.01ng/rxn).

Target gene: human GAPDH                     

Square Line: AddBioMeditek, Linear Line: Competitive A

AddProbe RT-qPCR Kit & RT-qPCR Master (2x conc.) with UDG

for TaqMan Probe

For real-time PCR with TaqMan Probe

UDG (Uracil-DNA Glycosylase) for prevention of carryover contamination from amplification products is included in         

    AddProbe RT-qPCR Kit and AddProbe RT-qPCR Master.

All components for real-time PCR is included – hot-start Taq DNA Polymerase, UDG, reaction buffer, dNTP mixture and

   PCR enhancer.

Applications: Real-time PCR, Quantitative real-time PCR, Infectious Disease detection

RT-qPCR Kit - multiplex test.png

Multiplex RT-qPCR test with 3 kinds of viral RNA  

Red Line: Cy5, Blue Line: FAM, Green Line: HEX    

RT-qPCR Kit -  UDG activity test.png

 UDG activity test between with UDG and without UDG

 through 1st- and 2nd- RT-qPCR test with human GAPDH

AddGenotype qPCR Master (2x conc.)

for TaqMan Probe

Optimized qPCR Master for genotyping by TaqMan Probe based qPCR.

All components is included for qPCR.

Hot-start Taq DNA Polymerase, reaction buffer, dNTP mixture, PCR enhancer are included in AddGenotype qPCR Master.

Applications: Genotyping Assay, SNP Analysis, Real-time PCR

Genotype qPCR
AddGreen qPCR Master (2x conc.)

for Intercalating Dye

Intercalating dye based real-time PCR for DNA target.

All components for qPCR is mixed except for primer and template DNA.

Hot-start Taq DNA Polymerase, reaction buffer (with 3 mM MgCl2), 2 mM dNTP mixture, PCR enhancer,

    Intercalating dye are included in AddGreen qPCR Master.

Applications: Quantitative real-time PCR, Gene expression analysis, Genetic variation analysis

AddGreen RT-qPCR Kit & RT-qPCR Master (2x conc.)

for Intercalating Dye

Intercalating dye based one-step real-time RT-PCR for RNA target.

All components for RT-qPCR is mixed except for primer and template DNA.

Hot-start Taq DNA Polymerase, Reverse Transcriptase, RNase Inhibitor, reaction buffer (with 3 mM MgCl2),

   2 mM dNTP mixture, PCR enhancer, intercalating dye are included in AddGreen RT-qPCR Kit & Master.

Applications: Quantitative real-time PCR, Gene expression analysis, Genetic variation analysis

Ordering Information
Product Name
Cat. No.
Size

AddProbe qPCR Master

with UDG (2x conc.)

72401U

5 mL

Supplied with

AddProbe qPCR Master with UDG (2x conc.)

1.0 mL x 5 tubes

AddProbe RT-qPCR Kit

with UDG

74201

100 rxn.

1. 20x Enzyme Solution 0.1 mL

2. 5x Reaction Buffer 0.4 mL

AddProbe RT-qPCR Master

with UDG (2x conc.)

74201U

1 mL

AddProbe RT-qPCR Master with UDG (2x conc.) 1.0 mL

AddGenotype qPCR Master (2x conc.)

78520

5 mL

AddGenotype qPCR Master (2x conc.) 1.0 mL x 5 tubes

AddGreen qPCR Master (2x conc.)

72501

5 mL

AddGreen qPCR Master (2x conc.) 1.0 mL

AddGreen RT-qPCR Kit

71202

100 rxn.

1. 20x Enzyme Solution 0.1 mL

2. 5x Reaction Buffer 0.4 mL

AddGreen RT-qPCR Master (2x conc.)

71302

1 mL

AddGreen RT-qPCR Master (2x conc.) 1.0 mL

Documents

Manual for AddProbe qPCR Master with UDG

Manual for AddProbe RT-qPCR Kit & Master with UDG

Manual for AddGenotype qPCR Master

Manual for AddGreen qPCR Master

Manual for AddGreen RT-qPCR Kit & Master