AddMedi SARS-CoV-2 Sequencing Kit
Full S gene Detection of SARS-CoV-2 Virus for Sequencing Through One-step RT-PCR
from Huamn Respiratory Samples
AddMedi SARS-CoV-2 Sequencing Kit
Full S gene Detection of SARS-CoV-2 Virus for Sequencing Through One-step RT-PCR
from Huamn Respiratory Samples
AddMedi SARS-CoV-2 Sequencing Kit
Full S gene Detection of SARS-CoV-2 Virus for Sequencing Through One-step RT-PCR
from Huamn Respiratory Samples
Reagents for Real-time PCR
with TaqMan Probe & Intercalating Dye
Research Use Only. Not for Use in Diagnostic Procedures.
for TaqMan Probe
● For real-time PCR with TaqMan Probe
● UDG (Uracil-DNA Glycosylase) for prevention of carryover contamination from amplification products is included in AddProbe qPCR Master.
● All components for real-time PCR is included – hot-start Taq DNA Polymerase, UDG, reaction buffer, dNTP mixture and PCR enhancer.
● Applications: Real-time PCR, Quantitative real-time PCR, Infectious Disease detection
Test of Limit of Detection (LOD) between “AddBioMeditek” and “Competitive A qPCR Product” with 10-fold serial dilutions of human genomic DNA (10, 1, 0.1 and 0.01ng/rxn).
Target gene: human B2M
Square Line: AddBioMeditek, Linear Line: Competitive A
Test of Limit of Detection (LOD) between “AddBioMeditek” and “Competitive A qPCR Product” with 10-fold serial dilutions of human genomic DNA (10, 1, 0.1 and 0.01ng/rxn).
Target gene: human GAPDH
Square Line: AddBioMeditek, Linear Line: Competitive A
for TaqMan Probe
● For real-time PCR with TaqMan Probe
● UDG (Uracil-DNA Glycosylase) for prevention of carryover contamination from amplification products is included in
AddProbe RT-qPCR Kit and AddProbe RT-qPCR Master.
● All components for real-time PCR is included – hot-start Taq DNA Polymerase, UDG, reaction buffer, dNTP mixture and
PCR enhancer.
● Applications: Real-time PCR, Quantitative real-time PCR, Infectious Disease detection
Multiplex RT-qPCR test with 3 kinds of viral RNA
Red Line: Cy5, Blue Line: FAM, Green Line: HEX
UDG activity test between with UDG and without UDG
through 1st- and 2nd- RT-qPCR test with human GAPDH
for TaqMan Probe
● Optimized qPCR Master for genotyping by TaqMan Probe based qPCR.
● All components is included for qPCR.
● Hot-start Taq DNA Polymerase, reaction buffer, dNTP mixture, PCR enhancer are included in AddGenotype qPCR Master.
● Applications: Genotyping Assay, SNP Analysis, Real-time PCR
for Intercalating Dye
● Intercalating dye based real-time PCR for DNA target.
● All components for qPCR is mixed except for primer and template DNA.
● Hot-start Taq DNA Polymerase, reaction buffer (with 3 mM MgCl2), 2 mM dNTP mixture, PCR enhancer,
Intercalating dye are included in AddGreen qPCR Master.
● Applications: Quantitative real-time PCR, Gene expression analysis, Genetic variation analysis
for Intercalating Dye
● Intercalating dye based one-step real-time RT-PCR for RNA target.
● All components for RT-qPCR is mixed except for primer and template DNA.
● Hot-start Taq DNA Polymerase, Reverse Transcriptase, RNase Inhibitor, reaction buffer (with 3 mM MgCl2),
2 mM dNTP mixture, PCR enhancer, intercalating dye are included in AddGreen RT-qPCR Kit & Master.
● Applications: Quantitative real-time PCR, Gene expression analysis, Genetic variation analysis
Ordering Information
AddProbe qPCR Master
with UDG (2x conc.)
72401U
5 mL
AddProbe qPCR Master with UDG (2x conc.)
1.0 mL x 5 tubes
AddProbe RT-qPCR Kit
with UDG
74201
100 rxn.
1. 20x Enzyme Solution 0.1 mL
2. 5x Reaction Buffer 0.4 mL
AddProbe RT-qPCR Master
with UDG (2x conc.)
74201U
1 mL
AddProbe RT-qPCR Master with UDG (2x conc.) 1.0 mL
AddGenotype qPCR Master (2x conc.)
78520
5 mL
AddGenotype qPCR Master (2x conc.) 1.0 mL x 5 tubes
AddGreen qPCR Master (2x conc.)
72501
5 mL
AddGreen qPCR Master (2x conc.) 1.0 mL
AddGreen RT-qPCR Kit
71202
100 rxn.
1. 20x Enzyme Solution 0.1 mL
2. 5x Reaction Buffer 0.4 mL
AddGreen RT-qPCR Master (2x conc.)
71302
1 mL
AddGreen RT-qPCR Master (2x conc.) 1.0 mL
Documents
Manual for AddProbe qPCR Master with UDG
Manual for AddProbe RT-qPCR Kit & Master with UDG
Manual for AddGenotype qPCR Master
Manual for AddGreen qPCR Master
Manual for AddGreen RT-qPCR Kit & Master