Hot-start Taq DNA Polymerase & Master Mix.

Research Use Only. Not for Use in Diagnostic Procedures.

AddStart Taq DNA Polymerase

AddStart Taq DNA Polymerase from recombinant E. coli.

AddStart Taq DNA Polymerase is hot-start Taq DNA Polymerase by specific anti-Taq monoclonal antibody.

It has 5’ → 3’ exonuclease activity and A-tailing in 3’ end of amplicon.

Normal maximum amplicon size is ~ 5 kb.

The polymerization rate is 1 kb per 1 min., but maximum rate is 2 kb per 1 min.

All components for PCR is included – Taq DNA Polymerase, 10x reaction buffer, 10 mM dNTP mixture

20 mM MgCl2 is included in 10x reaction buffer.

The concentration of AddStart Taq DNA Polymerase is 5.0 units/μl.

Applications: Real-time PCR, General PCR, Colony PCR, Screening PCR and Cloning

AddStart Taq DNA Master (2x conc.) with or without UDG

All components for PCR is mixed except for primer and template DNA.

UDG (Uracil-DNA Glycosylase) for prevention of carryover contamination from amplification products is included

    in AddStart Taq DNA Master with UDG (Cat. No. 36101U) product.

Hot-start Taq DNA Polymerase, reaction buffer (with 4 mM MgCl2), 4 mM dNTP mixture, PCR enhancer,

    loading dye (Orange G), and sediment for electrophoresis are included in AddStart Taq DNA Master.

The concentration of AddStart Taq DNA Master is 2x.

Applications: Hot-start PCR, General PCR, Colony PCR, Screening PCR and Cloning

AddMulti Taq DNA Master (2x conc.) with or without UDG

● UDG (Uracil-DNA Glycosylase) for prevention of carryover contamination from amplification products is included

    in AddMulti Taq DNA Master with UDG (Cat. No. 37101U) product.

● All components for PCR is mixed except for primer and template DNA.

● Hot-start Taq DNA Polymerase, reaction buffer (with 6 mM MgCl2), 6 mM dNTP mixture, PCR enhancer,

    loading dye (Orange G), and sediment for electrophoresis are included in AddMulti Taq DNA Master.

● The concentration of AddMulti Taq DNA Master is 2x.

● Applications: Multiplex PCR, General PCR, Colony PCR, Screening PCR and Cloning

Ordering Information
Product Name
Cat. No.
Size

AddStart Taq DNA Polymerase

18500

500 Units

AddStart Taq DNA Master (2x conc.)

36101

5 mL

Supplied with

1. AddStart Taq DNA Polymerase (5 U/ul) 100 uL

2. 10x Reaction Buffer 1.0 mL

3. 10 mM dNTP Mixture 1.0 mL

AddStart Taq DNA Master (2x conc.) 1.0 mL x 5 tubes

AddStart Taq DNA Master with UDG

(2x conc.)

36101U

5 mL

AddStart Taq DNA Master with UDG (2x conc.)

1.0 mL x 5 tubes

AddMulti Taq DNA Master (2x conc.)

37101

5 mL

AddMulti Taq DNA Master (2x conc.) 1.0 mL x 5 tubes

AddMulti Taq DNA Master with UDG

(2x conc.)

37101U

5 mL

AddMulti Taq DNA Master with UDG (2x conc.)

1.0 mL x 5 tubes

Documents

Manual for AddStart Taq DNA Polymerase

Manual for AddStart Taq DNA Master

Manual for AddMulti Taq DNA Master