Normal Taq DNA Polymerase & Master Mix.

Research Use Only. Not for Use in Diagnostic Procedures.

Add Taq DNA Polymerase

Normal Taq DNA Polymerase from recombinant E. coli.

It has 5’ → 3’ exonuclease activity and A-tailing in 3’ end of amplicon.

Normal maximum amplicon size is ~ 5 kb.

The polymerization rate is 1 kb per 1 min., but maximum rate is 2 kb per 1 min.

All components for PCR is included – Taq DNA Polymerase, 10x reaction buffer, 10 mM dNTP mixture

20 mM MgCl2 is included in 10x reaction buffer.

The concentration of Add Taq DNA Polymerase is 5.0 units/μl.

Applications: General PCR, Colony PCR, Screening PCR and Cloning

Add Taq DNA Master (2x conc.)

All components for PCR is mixed except for primer and template DNA.

Normal Taq DNA Polymerase, reaction buffer (with 4 mM MgCl2), 4 mM dNTP mixture, PCR enhancer,

   loading dye (Orange G), and sediment for electrophoresis are included in Add Taq DNA Master.

The concentration of Add Taq DNA Master is 2x.

The specification of Add Taq DNA Master is same with Add Taq DNA Polymerase as mentioned above.

AddTaq lambda DNA test

PCR was performed with 10pg lambda DNA and specific primer (amplicon size: 549bp ~ 2043bp) by Add Taq DNA Polymerase.

The PCR condition is as follows: 95℃ for 5 min, followed by 30cycles of 95℃ for 30 sec, 60℃ for 30sec and 72℃ for 1min. A final 5 min elongation step was performed at 72℃.

AddTaq human DNA test

PCR was performed with 1ng human genomic DNA and specific primer (amplicon size: 500bp ~ 1900bp) by Add Taq DNA Polymerase.

The PCR condition is as follows: 95℃ for 5 min, followed by 30cycles of 95℃ for 30 sec, 60℃ for 30sec and 72℃ for 1min. A final 5 min elongation step was performed at 72℃.

Ordering Information
Product Name
Cat. No.
Size

Add Taq DNA Polymerase

17500

500 Units

Supplied with

1. Add Taq DNA Polymerase (5 U/ul) 100 uL

2. 10x Reaction Buffer 1.0 mL

3. 10 mM dNTP Mixture 1.0 mL

Add Taq DNA Master (2x conc.)

35001

5 mL

Add Taq DNA Master (2x conc.) 1.0 mL x 5 tubes

Documents

Manual for Add Taq DNA Polymerase

Manual for Add Taq DNA Master